Part:BBa_K3619002:Design

cDNA encoding the protein glutathione S-transferase (GST)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 156
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence is codon optimized for expression in E. coli K12.

Source

The sequence was retrieved from reversed translation and for E. coli K12 of the Uniprot record sequence of Q1RBG0.

References

1. https://doi.org/10.1016/S0027-5107(01)00206-8

2. https://doi.org/10.1002/0471140864.ps0606s52

3. https://doi.org/10.1016/j.pep.2017.08.003

4. https://www.uniprot.org/uniprot/Q1RBG0